Quantitative genomic RNA from Severe acute respiratory syndrome-related coronavirus 2 strain USA/MD-HP01542/2021 (variant B.1.351)

Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/MD-HP01542/2021.

This isolate is lineage B.1.351, clade GH according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.

The following mutations are present in the clinical isolate: Spike A243del, Spike A701V, Spike D80A, Spike D215G, Spike D614G, Spike E484K, Spike K417N, Spike L18F, Spike L242del, Spike L244del, Spike N501Y, E (Envelope protein) P71L, N (Nucleocapsid protein) T205I, NSP3 (Non-structural protein 3) G172C, NSP3 Q57H, NSP3 S171L, NSP2 (Non-structural protein 2) T85I, NSP3 K837N, NSP5 (Non-structural protein 5) K90R, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L. One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.

Genomic RNA from SARS-CoV-2 must be handled at BSL-2 containment.

The whole-genome sequence of this strain is available on the ATCC Genome Portal.

Acknowledgment for publications should read "The following reagent was deposited by the Centers for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH: Genomic RNA from SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP01542/2021 (Lineage B.1.351), NR-55349."

1. Thaw the vial on ice. Avoid exposing the RNA to repeated freeze-thaw cycles as it may result in degradation.
2. Gently mix the sample to ensure an even distribution of material.
3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.

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