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Heat-inactivated SARS-CoV-2 variant B.1.1.7

VR-3326HK

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This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/CA_CDC_5574/2020 that has been inactivated by heating.

Product category
Viruses
Product type
Animal virus
Inactivated organism
Classification
Coronaviridae, Betacoronavirus, Severe acute respiratory syndrome-related coronavirus 2
Strain designation
USA/CA_CDC_5574/2020
Common name
SARS-CoV-2
Genome sequenced strain
Yes
Isolation source
Nasopharyngeal swab, San Diego County, CA, December 29, 2020.
Geographical isolation
United States; California
Applications
Assay development
Infectious disease research
Respiratory disease research
Product format
Frozen
Shipping information
Each vial contains approximately 0.25 mL of heat-inactivated, clarified cell lysate and supernatant from Vero cells infected with SARS-CoV-2 strain USA/CA_CDC_5574/2020. The vial should be centrifuged prior to opening.
Storage conditions
-70°C or colder
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Price: $1,200.00 ea
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Assay development
Assay control
Research
This product is intended for use in molecular assays or as a process control (e.g., viral RNA extraction through qPCR amplification process). ATCC VR-3326HK can also be used to make a dilution series to estimate level of detection.

Characteristics

Comments

Due to high demand, this product is limited to 20 per organization per 60 days.

This isolate is lineage B.1.1.7, clade GR according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.

This isolate has been sequenced in the ATCC Genome Portal.

The following mutations were identified in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, M (Membrane protein) V70L, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NS3 T223I, NS8 (Non-structural protein 8) Q27stop, NS8 R52I, NS8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) A454V, NSP13 K460R.

One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

Handling information

Handling notes

Due to high demand, this product is limited to 20 per organization per 60 days.

This isolate is lineage B.1.1.7, clade GR according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.

The following mutations were identified in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, M (Membrane protein) V70L, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NS3 T223I, NS8 (Non-structural protein 8) Q27stop, NS8 R52I, NS8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) A454V, NSP13 K460R.

One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.

Each vial contains approximately 0.25 mL of heat-inactivated, clarified cell lysate and supernatant from Vero cells infected with SARS-CoV-2 strain USA/CA_CDC_5574/2020. The vial should be centrifuged prior to opening.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

Quality control specifications

Volume
250 μL
Verification method
Whole-genome Sequencing

History

Depositors
CDC
Year of origin
2020

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

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