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Enterovirus A71

VR-1775

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Enterovirus A71 strain BrCr is propagated in Vero cells (ATCC CCL-81). This strain was originally isolated from the stool specimen of a 2-month-old male with aseptic meningitis and was derived by passage in Vero cells. It has applications in enteric disease research.
Product category
Viruses
Product type
Animal virus
Classification
Enterovirus, Enterovirus A, Enterovirus A71
Strain designation
BrCr
Genome sequenced strain
Yes
Isolation source
Stool specimen from 2-month-old male with aseptic meningitis. Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product replaces ATCC VR-784. and exhibits CPE when cells are infected.
Applications
Enteric disease research
Infectious disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Enteric Research

Characteristics

Comments
Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product replaces ATCC VR-784 and exhibits CPE when cells are infected.
Note: The sequences of ATCC VR-784 and ATCC VR-1775 have not been compared.
Mycoplasma contamination
Not detected

Handling information

Host
Effects
CPE; cell degeneration; cell rounding; cell sloughing
Complete medium
EMEM (ATCC 20-2003) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
Incubate infected culture for 5-9 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Recommendation for infection
Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Derived by passage of ATCC VR-784 in Vero cells (ATCC CCL-81). This product will replace ATCC VR-784.  (Note:  The sequences of ATCC VR-784 and ATCC VR-1775 have not been compared).  
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
CPE, Cytopathic effect
MOI, Multiplicity of infection

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
NJ Schmidt
Chain of custody
Deposited as Enterovirus type 71
Type of isolate
Human
Patient age
2 months
Patient gender
Male

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245

Frequently Asked Questions

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