• Quick Order

Human respiratory syncytial virus, High titer


Download Genome Learn about our Enhanced Authentication Initiative
Human respiratory syncytial virus strain 18537 is a high-titer preparation that is propagated in HEp-2 cells (ATCC CCL-23). This strain was isolated in 1962 from the respiratory secretions of a child with acute respiratory disease seen at Children’s Hospital in Washington, DC, and was deposited by the National Institute of Allergy and Infectious Diseases (NIAID). It has applications in virucide testing, respiratory research, assay development and vaccine development.
Product category
Product type
Animal virus
Purified virus
Pneumoviridae, Orthopneumovirus, Human orthopneumovirus
Strain designation
Isolation source
Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC. Treated ATCC VR-1401 to removed Mycoplasma at ATCC.
Geographical isolation
United States; Washington, DC
Assay development
Infectious disease research
Respiratory disease research
Product format
Storage conditions
-70°C or colder
Buy Now
Price: $974.00 ea
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days


Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral studies
Spiking studies
ELISpot assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development



ATCC VR-1580PQ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC VR-1580 via concentration through a sucrose cushion.

The early passage history of this item is unknown.

Handling information

CPE; cell rounding; cell sloughing; syncytia
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
95% Air, 5% CO2
3-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes

ATCC VR-1580PQ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC VR-1580 via concentration through a sucrose cushion.

The early passage history of this item is unknown.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EDTA, Disodium ethylene-diaminetetraacetate
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection

Quality control specifications

Specification range
>107 TCID50/mL
0.5 mL
Verification method
Whole-genome Sequencing


Deposited as
Respiratory syncytial virus
RM Chanock, National Institute of Allergy and Infectious Diseases; ATCC
Type of isolate
Year of origin
Patient age

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.



US and Puerto Rico


Outside the US


Hours of Operation

9:30am - 5:30pm
US Eastern Time