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Betacoronavirus 1


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Betacoronavirus 1 strain OC43 is propagated in HCT-8 [HRT-18] cells (ATCC CCL-244). This strain was isolated from a man with a cold-like illness. It has applications in respiratory disease research and infectious disease research.
Product category
Product type
Animal virus
Coronaviridae, Betacoronavirus
Strain designation
Common name
Human coronavirus OC43
Isolation source
Man with cold-like illness. Treated to remove mycoplasma at ATCC.
Infectious disease research
Respiratory disease research
Product format
Storage conditions
-70°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Respiratory research


ATCC VR-1558 is a TC-adapted item derived from virus passaged in mice (ATCC VR-759).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC VR-740).

Next-generation sequencing (NGS) at ATCC on the HCT-8 [HRT-18] cell line (ATCC CCL-244) used as the host has shown the presence of a putative fragment of Gammaherpesvirus with a ≥98% homology to Human Heparanase 2 (NCBI number NG_023416.1).
Mycoplasma contamination
Not detected

Handling information

HCT-8 [HRT-18] (ATCC CCL-244)
CPE; cell vacuolization; cell sloughing
Complete medium
RPMI-1640 (ATCC 30-2001) + 2% HS (ATCC 30-2040)
4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Handling notes
ATCC VR-1558 is a TC-adapted item derived from virus passaged in mice (ATCC VR-759).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC VR-740).
Key abbreviations
RPMI, Roswell Park Memorial Institute
TC, Tissue culture
CPE, Cytopathic effect
HCT-8, adult human colon epithelial cells
HS, Horse serum
MOI, Multiplicity of infection
MRA, Mycoplasma removal agent
PBS, Phosphate-buffered saline

Quality control specifications

1.0 mL
Verification method
Whole-genome Sequencing


Deposited as
Human coronavirus OC43
JO Fleming, University of Wisconsin, Madison
Chain of custody
ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Type of isolate
Patient gender
Cross references
GenBank NC_005147.1 Human coronavirus OC43, complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356

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