AKT Genetic Alteration Cell Panel

TCP-1029 ^™

AKT is a serine–threonine protein kinase that is expressed as three isoforms (AKT1, -2 and -3). AKT activation is initiated by translocation to the plasma membrane, which is mediated by a receptor tyrosine kinase-PI3K pathway. Activated AKT phosphorylates many key proteins, such as glycogen synthase kinase 3 and FOXOs, and regulates cell survival, proliferation and other cellar processes. Amplification of AKT1 and AKT2 has been discovered in a variety of common tumor types. AKT1 is linked to tumor cell survival and growth, whereas AKT2 is linked to tumor invasiveness.

The AKT genetic alteration cell panel (ATCC TCP-1029) is composed of eight human tumor cell lines from common cancer types that carry ATK gene copy number changes. The AKT1 and AKT2 gene alteration status of each cell line has been sequenced and validated by ATCC. This panel is useful for AKT pathway research, as well as for developing pan-AKT inhibitors or isoform-specific AKT inhibitors for anti-cancer therapeutics.

Components: ATCC CRL-2321 HCC1143
ATCC CRL-7245 Hs 343.T
ATCC CRL-1469 PANC-1
ATCC HTB-161 NIH:OVCAR-3
ATCC CRL-1622 KLE
ATCC HTB-183 NCI-H661
ATCC HTB-20 BT-474
ATCC HTB-128 MDA-MB-415
Product format: Frozen
Storage conditions: Vapor phase of liquid nitrogen

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Product sheet

Instructions for use

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: This panel is useful for AKT pathway research, as well as for developing pan-AKT inhibitors or isoform-specific AKT inhibitors as anti-cancer therapeutics.

Characteristics

Comments: AKT is a serine–threonine protein kinase that is expressed as three isoforms (AKT1, -2 and -3). AKT activation is initiated by translocation to the plasma membrane, which is mediated by a receptor tyrosine kinase-PI3K pathway. Activated AKT phosphorylates many key proteins, such as glycogen synthase kinase 3 and FOXOs, and regulates cell survival, proliferation and other cellar processes. Amplification of AKT1 and AKT2 has been discovered in a variety of common tumor types. AKT1 is linked to tumor cell survival and growth, whereas AKT2 is linked to tumor invasiveness.

The AKT genetic alteration cell panel (ATCC TCP-1029) is composed of eight human tumor cell lines from common cancer types that carry ATK gene copy number changes. The AKT1 and AKT2 gene alteration status of each cell line has been sequenced and validated by ATCC. This panel is useful for AKT pathway research, as well as for developing pan-AKT inhibitors or isoform-specific AKT inhibitors for anti-cancer therapeutics.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

For-profit Research Use License from MD Anderson Cancer Center

For-profit Organizations: For every order of this item, you must work directly with the contributor, MD Anderson Cancer Center to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from MD Anderson Cancer Center that we are authorized to ship each order.

We are providing the following contact information, but this information may change without notice:
M.D. Anderson Cancer Center
Office of Technology Commercialization
Email: [email protected]

Once ATCC has received authorization from MD Anderson Cancer Center, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

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References

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