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Trypanosoma brucei brucei

PRA-377

Product category
Protists
Product type
Parasitic protozoan
Strain designation
TREU 927/4 (GUTat 10.1)
Genome sequenced strain
Yes
Isolation source
Clone derived from GPAL/KE/70/EATRO 1534 isolate, tsetse fly
Geographical isolation
Kenya
Applications
Agricultural research
Infectious disease research
Vector-borne disease research
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

Characteristics

Comments
Genome strain

Handling information

Medium
Instruction for complete medium
Media: ATCC® Medium 2831: SDM-79 Medium

Alternate Media: ATCC® Medium 431: Trypanosome medium
Temperature
27°C
Handling procedure
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.
  1. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min). Immerse the ampule just sufficiently to cover the frozen material.  Do not agitate the ampule.
  2. Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing 10.0 mL of growth medium. Incubate at 27°C with the cap screwed on tightly.
Culture maintenance
  1. Agitate a culture at or near peak density and aseptically transfer 0.5-1 mL to a new tissue culture flask with fresh growth medium.
  2. Incubate at 27°C with the cap screwed on tightly.
  3. Transfer the culture every 3-7 days as described in steps 1-2.  The transfer interval will depend on the quantity of the inoculum and the quality of the medium.  This should be empirically determined by examining the culture on a daily basis until the growth cycle has stabilized.
Cryopreservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 0.5–1.0 x 107/mL in fresh growth medium.  If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 20% (v/v) solution of sterile glycerol in fresh growth medium. 
  4. Mix the cell preparation and the glycerol solution in equal portions. The final concentration will be 2.5-5 x 106 cells/mL in 10% glycerol. The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.  At -40ºC, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after it is thawed.
  9. Remove the vial from the water bath immediately after thawing.  Aseptically transfer the contents of the ampule into 10 mL of fresh growth medium. 
  10. Incubate the flask at 27°C with the cap screwed on tightly.
  11. Maintain as described above. 

History

Depositors
J Donelson
Chain of custody
ATCC <-- J Donelson
Type of isolate
Arthropod
Year of origin
1970
Cross references
GenBank AL929603 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 1, complete sequence
GenBank AE017150 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 2, complete sequence
GenBank CP000066 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 3, complete sequence
GenBank CP000067 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 4, complete sequence
GenBank CP000068 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 5, complete sequence
GenBank CP000069 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 6, complete sequence
GenBank CP000070 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 7, complete sequence
GenBank CP000071 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 8, complete sequence
GenBank CM000207 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 9, whole genome shotgun sequence
GenBank CM000208 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 10, whole genome shotgun sequence
GenBank CH464492 Trypanosoma brucei brucei strain 927/4 GUTat10.1 chromosome 11 chr11_scaffold02 genomic scaffold, whole genome shotgun sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Goedbloed E, et al. Serological studies of trypanosomiasis in East Africa. II. Comparisons of antigenic types of Trypanosoma brucei subgroup organisms isolated from wild tsetse flies. Ann Trop med Parasitol 67(1): 31-43, 1973. PubMed: 4723213

Gibson W. The origins of the trypanosome genome strains Trypanosoma brucei brucei TREU 927, T. b. gambiense DAL 972, T. vivax Y486 and T. congolense IL3000. Parasit Vectors 5: 71, 2012. PubMed: 22483376

van Deursen FJ, et al. Characterisation of the growth and differentiation in vivo and in vitro-of bloodstream-form Trypanosoma brucei strain TREU 927. Mol BIochem Parasitol 112(2): 163-171, 2001. PubMed: 11223123

Berriman M, et al. The genome of the African trypanosome Trypanosoma brucei. Science 309(5733): 416-422, 2005. PubMed: 16020726

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