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0.1% Gelatin Solution

PCS-999-027

0.1% Gelatin Solution is used to coat cell culture flasks. The use of coated-flasks improves cell attachment for certain types of primary cells as well as certain continuous cell lines.
Formulation: 0.1% porcine gelatin in water. 
Product category
Reagents
Comments
The use of coated flasks improves cell attachment for certain types of primary cells as well as certain continuous cell lines. This sterile-filtered solution contain 0.1% porcine gelatin in water.
Applications
Cell growth and viability
Volume
100 mL
Storage conditions
2°C to 8°C
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Documentation

ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
0.1% Gelatin Solution is used to coat cell culture flasks.

Handling information

Handling procedure

For optimal cell attachment, cell culture flasks should be prepared before thawing the cells.

  1. Decontaminate the external surface of the 0.1% Gelatin Solution bottle by spraying with 70% ethanol.
  2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, add 1.0 mL of 0.1% Gelatin Solution per 10 cm2 of culture surface area (e.g., 2.5 mL if using a T-25 flask). 
  3. Rock culture flask to coat surface; place in a 37°C incubator (with or without 5% CO2) for at least 30 minutes, and up to overnight.
  4. Aspirate the excess gelatin solution from the culture flasks using sterile technique.
  5. Add 5.0 mL of complete growth medium per 25 cm2 of culture surface area (e.g., 5.0 mL if using a T-25 flask).
  6. Place the gelatin-coated flasks in a 37°C, 5% CO2 incubator for at least one hour to equilibrate before inoculating with the cell suspension.   
Cryopreservation
Storage: Store the 0.1% Gelatin Solution at 2°C to 8°C. When stored as directed, the product is stable until the expiration date on the label.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Functional tests
Each lot is assessed for acceptable attachment of human primary cells.
A Certificate of Analysis (COA) is available upon request for each lot of 0.1% Gelatin Solution.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Frequently Asked Questions

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Telephone

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