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ABRF-MGRG 10 Strain Staggered Mix Genomic Material


NGS Standards are mock microbial communities that mimic mixed metagenomic samples. This product comprises genomic DNA from fully sequenced, characterized, and authenticated bacterial and archaeal species observed in various environments, including soil, freshwater, seawater, feces, and high salinity ecosystems.
Product category
Product type
Nucleic acid
NGS standard
Nucleic acids extracted from:
0.43% Bacillus subitilis (ATCC 23857)
42.88% Chromobacter violaceum (ATCC 12472)
0.04% Entercoccus faecalis (ATCC 47077)
0.43% Escherichia coli (ATCC 700926)
0.43% Halobacillus halophilus (ATCC 35676)
4.29% Haloferax volcanii (ATCC 29605)
4.29% Micrococcus luteus (ATCC 4698)
4.29% Pseudoalteromonas haloplanktis (ATCC 14393)
0.04% Pseudomonas fluorescens (ATCC 13525)
42.88% Staphylococcus epidermidis (ATCC 12228)
Microbiome research
Next-generation sequencing
Product format
Shipping information
Stored in Tris-HCl pH 8.5
Storage conditions
-20°C or colder
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Price: $224.00 ea
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
NGS Standards enable the optimization of metagenomics workflows and research applications, providing reliable comparative data while improving assay consistency.

This product is ideal for environmental analyses, assay development, and as a daily run control for molecular diagnostics and microbiome applications.


NGS Standards are mock microbial communities that mimic mixed metagenomic samples. The ABRF-MGRG metagenomics reference standard was developed and packaged in partnership with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Group (MMRG)*, an international scientific society dedicated to advancing technologies, education, communication, and reproducible research in the operations of shared scientific resources, as part of the Extreme Microbiome Project (XMP). This product comprises genomic DNA from fully sequenced**, characterized, and authenticated bacterial and archaeal species observed in various environments, including soil, freshwater, seawater, feces, and high salinity ecosystems. Further, this reference community was derived from Class I microbial genomes (Koren et al., Genome Biol 14(9):R101, 2013), was extracted to retain long fragments of gDNA, and is confirmed to be free of human DNA. Each order for this product is provided with access to run 20 analyses on One Codex, the leading bioinformatics platform for microbial genomics and metagenomics.

Specification Range: 4.25 ng/µL to 7.09 ng/µL***

*The bulk material for this product was deposited by ABRF-MGRG. The research group extracted DNA from each individual strain and mixed them in appropriate proportions.
**The reference genome for Pseudoalteromonas haloplanktis is not available.
***DNA concentrations indicate ATCC manufacturing specifications and are provided as a reference only.

Quality control specifications

50 μL


Special collection
NGS Standards

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Purchase of this product will include limited use access to the One Codex platform for metagenomic analysis of ATCC microbiome products. By purchasing this product, you consent and agree to allow ATCC to securely transfer sales and end users’ data related to this product to One Codex. Provision of One Codex access, storage, management of sequencing data, and the interpretation and accuracy of analytical results provided by One Codex are the responsibility of One Codex and not ATCC. End users should visit for their complementary metagenomic data analysis.

One Codex

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


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Outside the US

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