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Pseudomonas fluorescens Migula

13525

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This is a bacterial type strain that is recommended for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.  This strain is also used to assay antimicrobial preservatives.
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
NCTC 10038 [28/5, CCEB 546, DSM 50090, NCIB 9046, NCPPB 1964, PJ239, R. Hugh 818, R.Y. Stanier 192, Biotype A]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Pre-filter tanks
Geographical isolation
England
Applications
Bioinformatics
Water testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Assay of antimicrobial preservatives
Reference material
Water testing
Preceptrol
Yes

Characteristics

Comments
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Open the vial according to the enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the primary broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate the tubes and plate at 26°C for 24-48 hours.
Handling notes
This strain produces fluorescein pigment.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Pseudomonas fluorescens Migula
Depositors
NCTC
Chain of custody
ATCC <-- NCTC <-- ME Rhodes 28/5
Cross references
GenBank NC_016830.1 Pseudomonas fluorescens F113, complete genome
GenBank AZ254357 Z9 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic, genomic survey sequence
GenBank D86033 Pseudomonas putida rpoD gene for RNA polymerase sigma-70 factor, partial cds, strain: IAM 12022.
GenBank AF207608 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C2, genomic survey sequence
GenBank L24790 Pseudomonas fluorescens (ATCC 13525) ribosomal RNA gene fragment.
GenBank L13747 Pseudomonas fluorescens p-hydroxy benzoate hydroxylase gene, complete cds.
GenBank D86001 Pseudomonas putida 16S ribosomal RNA gene, strain: IAM 12022, partial sequence.
GenBank AF056495 Pseudomonas fluorescens glutaminase-asparaginase precursor, gene, complete cds.
GenBank AF094725 Pseudomonas fluorescens strain ATCC 13525 16S ribosomal RNA gene, partial sequence.
GenBank D86016 Pseudomonas putida gyrB gene for DNA gyrase B subunit, partial cds, strain: IAM 12022.
GenBank AF207609 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C4, genomic survey sequence
GenBank AF207610 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C6, genomic survey sequence
GenBank AF207611 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C7, genomic survey sequence
GenBank AF207612 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C8, genomic survey sequence
GenBank AF207613 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C9, genomic survey sequence
GenBank AF207614 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C10, genomic survey sequence
GenBank AF207615 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C11, genomic survey sequence
GenBank AF207616 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C13, genomic survey sequence
GenBank AF207617 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C15, genomic survey sequence
GenBank AF207618 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C16, genomic survey sequence
GenBank AF207619 Pseudomonas fluorescens ATCC 13525 Pseudomonas fluorescens genomic clone C17, genomic survey sequence
GenBank AZ254352 Z1 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic similar to P-type ATPase, genomic survey sequence
GenBank AZ254353 Z2 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic similar to pyoverdine biosynthesis, genomic survey sequenc
GenBank AZ254354 Z3 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic similar to pyoverdine biosynthesis, genomic survey sequence
GenBank AZ254355 Z5 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic similar to pyoverdine biosynthesis, genomic survey sequence
GenBank AZ254356 Z6 Pseudomonas transposon mutant bank Pseudomonas fluorescens genomic similar to pyoverdine biosynthesis, genomic survey sequence
GenBank AY007258 Pseudomonas fluorescens ATCC 13525 CztD (cztD) gene, partial cds; CztC (cztC), CztB (cztB), CztA (cztA), CztS (cztS), CztR (cztR), CztO (cztO), and ORF1 genes, complete cds; and methyl-accepting chemoreceptor-like protein ORF2 gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Water microbiology. Method 10: Pseudomonads--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.10-1995.

Water microbiology. Method 11: Pseudomonads -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.11-1995.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

. Opinion 37. Designation of strain ATCC 13525 as the neotype strain of Pseudomonas fluorescens Migula. Int. J. Syst. Bacteriol. 20: 17-18, 1970.

Stanier RY, et al. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43: 159-271, 1966. PubMed: 5963505

View All Curated Citations for this Product

Frequently Asked Questions

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