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Leibovitz's L-15 medium (ATCC 30-2008) with 10 µg/ml insulin (Gibco Cat# 12585-014), 16 µg/ml glutathione (Sigma Cat# G6013 or equivalent), 90%; fetal bovine serum, 10%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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