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MEL167
 

CRL-3651

MEL167 is a suspension Circulating Tumor Cell (CTC) line derived from a patient undergoing treatment for metastatic melanoma. Melanoma CTCs shed from a primary tumor and circulate through the bloodstream increasing the risk for metastatic disease. Due to these metastatic characteristics, MEL167 is intended for the study of early diagnosis and monitoring of tumor progression for metastatic melanoma.
 
Product category
Human cells
Product type
Cell model
Organism
Homo sapiens, human
Cell type
epithelial-like cell
Morphology
Rounded with clusters
 
Tissue
Skin
Disease
Melanoma; Metastatic
Applications
Cancer research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Metastatic melanoma, disease progression, pre-clinical studies
 

Characteristics

Cells per vial
≥ 2.5 x 106
Volume
1.0 mL
Growth properties
Suspension
Age
49 years
Gender
Male
Clinical data
Derived from a patient undergoing treatment for metastatic melanoma.
 
Oncogene
BRAF(V600E), CDKN2A (R29*), STK11(C418W)
 
Comments
MEL167 is intended for the study of metastatic melanoma progression. This cell line was derived from a 49 year old male patient undergoing treatment for metastatic melanoma. The transition from primary melanoma to metastic disease offers new therapeutic targets.
 

Handling information

Complete medium

The base medium is Gibco™ RPMI with phenol red and GlutaMAX (ThermoFisher catalog # 61870-036). To make the complete growth medium, add the following components to the base medium: 

• Gibco™ B27 Supplement (50x) (ThermoFisher Scientific catalog #17504-044) to a final concentration of 2%.
• ATCC® Rock Inhibitor (Y-27632) (ATCC ACS-3030) to a final concentration of 100 nM.

Due to limited stability, at each sub-culture, the following components should be added to the complete medium:
• Gibco™ EGF (ThermoFisher Scientific catalog # PHG0311 or equivalent) to a final concentration of 20 ng/mL. 
• Gibco™ FGF (ThermoFisher Scientific catalog # PHG0261 or equivalent) to a final concentration of 20 ng/mL.

 
Temperature
37°C
Atmosphere
Hypoxic: 4% O2, 5% CO2
Handling procedure

To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70° C.  Storage at -70°C will result in loss of viability.  

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.   Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing   9.0 mL complete culture medium. and spin at approximately 300x g for 3 minutes.
  4. Resuspend cell pellet in 5 mL fresh pre-warmed complete growth medium, and transfer the cell suspension into a vented, Ultra-low attachment culture flask (Corning® Catalog # 4616 or 3814 )
  5. Incubate the culture at 37° C in a suitable incubator.  A 5% CO2 in 4% O2air atmosphere is recommended if using the medium described on this product sheet.
  6. It is recommended to subculture every 3 days or when the medium pH begins to change.

         

Subculturing procedure
Cultures can be maintained by centrifugation and can then be established by resuspending the cells in fresh medium at 2 X 105 viable cells/mL. Maintain cell density between 1.5 X 105 and 6 X 105 cells/mL.

Medium Renewal: Every 2 to 3 days (depending on medium pH).
Reagents for cryopreservation
ATCC Stem Cell Freezing Media (ATCC 30-2200)
 
Cryopreservation
Stem Cell Freeze Media (ATCC 30-2200)
 

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Virus testing
Not detected
Functional tests
NG2 and MLANA melanoma marker staining 
 

History

Depositors
Daniel Haber, MD, PhD
Massachusetts General Hospital
Cancer Center
149 13th St.
Charlestown, MA USA 02129
 
Year of origin
2016

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

Hong X, et al. The lipogenic regulator SREBP2 induces transferrin in circulating melanoma cells and suppresses ferroptosis. Cancer Discov Mar 11(3): 678-695, 2021. Doi: 10.1158/2159-8290.CD-19-1500. PMID: 33203734 PMCID: PMC7933049.

For product-related inquiries and issues, contact Product Experience:

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