Acetobacterium tundrae Simankova et al.
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2. If needed, exchange the gas in the test tube for 100% N2.
3. If the medium is pink (see discussion about resazurin) add 0.1 ml of reducing agent (1.5% sodium sulfide, stock solution) per each 5 to 6 ml of medium. Let the medium sit at room temperature for 10 to 20 minutes - until the resazurin becomes colorless - before inoculating.
4. When the Balch tube is ready to inoculate, open the vial according to enclosed instructions.
6. Growth is detected in the broth within 4 to 7 days. There should be no growth detected on the aerobic plate.
a. Balch tube refers to a special type of test tube that is designed to be pressurized and is suited for anaerobic work. The Balch test tubes can be purchased form Bellco glass (www.bellcoglass.com stock no. 2048-00150).
b. Resazurin is a commonly used redox indicator that is pink when the redox potential is above 50 mv., and colorless when the redox potential is below 110 mv. i.e. highly reducing. Most strict anaerobes require this low redox potential for optimum growth.
c. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added. Common reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate.
Cells are gram positive non-spore-forming motile rods. Optimum temperature is 20oC.
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Validation list no. 80. Int. J. Syst. Bacteriol. 51: 793-794, 2001.
Simankova MV, et al. Acetobacterium tundrae sp nov, a new psychrophilic acetogenic bacterium from tundra soil.. Arch. Microbiol. 174: 440-447, 2000. PubMed: 11195100
tundra wetland soil, Polar Ural