Nesiotobacter exalbescens Donachie et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #2 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 24 to 48 hours.
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hypersaline lake on the island of Laysan, Northwestern Hawaiian Islands
Stuart Donachie, personal communication
Donachie SP, et al. Nesiotobacter exalbescens gen. nov., sp. nov., a moderately thermophilic alphaproteobacterium from an Hawaiian hypersaline lake. Int. J. Syst. Evol. Microbiol. 56(Pt 3): 563-567, 2006. PubMed: 16514028