Geobacter grbiciae Coates et al.
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2. Under anaerobic conditions, rehydrate the cell pellet with a small amount of #2260 broth. Transfer this aliquot back into the tube of broth.
3. Additional #2260 broth tubes can also be inoculated with 0.5 ml from the original broth.
4. Incubate at 30oC for 3-5 days.
5. Growth is evident by slight turbidity in the broth.
This culture only grows in broth. Cells are Gram negative small rods.
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Coates JD, et al. Isolation of Geobacter species from diverse sedimentary environments. Appl. Environ. Microbiol. 62: 1531-1536, 1996.
Coates JD, et al. Geobacter hydrogenophilus, Geobacter chapellei and Geobacter grbiciae, three new, strictly anaerobic, dissimilatory Fe(III)-reducers. Int. J. Syst. Evol. Microbiol. 51: 581-588, 2001. PubMed: 11321104
freshwater aquatic sediment, Potomac River Estuary