Sulfitobacter brevis Labrenz et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 20oC for 5 days.
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Labrenz M, et al. Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., alpha-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int. J. Syst. Evol. Microbiol. 50: 303-313, 2000. PubMed: 10826817