Helicobacter pylori (Marshall et al.) Goodwin et al.

1. Open thawed vial.
2. Aseptically transfer the entire contents to a 5-6 mL tube of #1115 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
4. Or, to obtain a biphasic culture, add several drops of the primary broth tube to a #260 agar slant. Best practice is to incubate these slants at an angle.
5. Incubate at 37°C under microaerophilic conditions for 48 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.

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