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Pseudomonas aeruginosa (Schroeter) Migula

BAA-3255

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This drug-resistant bacterium was isolated in 2014 from the sputum of a 34-year-old male in Spain. This product can be used for all stages of the discovery and development process for novel antimicrobials and therapeutics, molecular-­based detection assays, and updated sterility protocols.
Product category
Bacteria
Product type
Drug-resistant bacterium
Strain designation
1124989
Type strain
No
Genome sequenced strain
Yes
Isolation source
Sputum
Geographical isolation
Spain
Applications
Antimicrobial resistance research
Bioinformatics
Drug development
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Drug discovery and development
Development of molecular-based detection assays
Development of updated sterility protocols
Preceptrol
No

Characteristics

Gram stain
Negative
Annotated genes
amrA
amrB
APH(3")-IIb
catB7
macB
mexA
mexB
mexC
mexD
mexE
mexF
mexH
mexI
mexK
mexN
mexP
mexQ
mexV
mexW
msbA
muxB
muxC
oprM
OXA-50
PDC-1
Susceptibility profile
Resistant
Ceftazidime
Ciprofloxacin
Doripenem
Imipenem
Levofloxacin
Meropenem
Piperacillin-tazobactam

Intermediate
Aztreonam
Cefepime

Susceptible
Amikacin
Colistin
Comments

The susceptibility profile information for this strain is initial characterization data acquired during the ATCC accessioning process.

Minimum inhibitory concentration (MIC) ranges for resistant, intermediate, and susceptible are based on criteria within the Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, 26th Edition.

Predicted antimicrobial resistance gene sequence data are provided for informational purposes only and are based on open reading frames (ORFs) identified and annotated from contigs produced during next-generation sequencing (NGS). ATCC does not warrant that the entire predicted antimicrobial resistance gene is present within the sequence prepared for lot-specific reports. Likewise, the information provided merely indicates that some portion of the sequence (whole or in part) is present based on analytical base pair alignment with known gene sequences.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open thawed vial.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #18 plate and/or #18 agar slant.
  4. Incubate at 37°C for 18-24 hours.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Pseudomonas aeruginosa
Depositors
International Health Management Associates (IHMA), Inc.
Chain of custody
ATCC <-- International Health Management Associates (IHMA), Inc.
Year of origin
2014
Patient age
34 years
Patient gender
Male

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. Note: These are considered live cultures, not diagnostic or analytics samples. We cannot ship this item until we receive this permit.

Permit to Move Live Plant Pests, Noxious Weeds, and Soil

For every order of this item, you must provide a valid Permit to Move Live Plant Pests, Noxious Weeds, and Soil (PPQ 526) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit. When requesting this permit, the USDA will require isolation information for this item, and you can find this information in the “Geographical isolation” and “Isolation source” fields on the respective product page. If you need assistance with determining the isolation information, please contact our Technical Services team or your applicable distributor.

Once you have the necessary permit, email the permit to [email protected] with a reference to both your account and sales order numbers. Once received, your permit will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, 26th Edition. Wayne, PA. M100S, 2016.

For product-related inquiries and issues, contact Technical Service:

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