Escherichia coli NanoLuc®
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
|Escherichia coli NanoLuc®||Frozen in glycerol||3 vials||-80°C or colder|
|Nano-Glo® Luciferase Assay Buffer||Frozen in glycerol||490 µL||-20°C or colder|
|Nano-Glo® Luciferase Assay Substrate||Frozen in glycerol||12 µL||-20°C or colder|
|Strain Propagation and Detection|
|Trypticase Soy Agar/Broth||Inoculation loop|
|Water bath||Eppendorf tubes|
|70% Ethanol||Cotton swab|
|37°C incubator||Laboratory paper (Whatman)|
|Pipette and tips||Centrifuge|
|Over-passage of the strain, resulting in loss of the plasmid.||Do not passage the strain more than 2 times.|
|The reaction solution is old or improperly prepared.||Discard. Prepare reaction solution with ≥5% substrate and 95% buffer. Mix well.|
|Inadequate amount of reaction solution used.||Ensure that your sample is fully saturated.|
|Contamination with another strain.||Discard the culture. Begin with a fresh culture.|
|Culture is not growing due to improper culture conditions.||Check that the culture conditions (medium, temperature, and atmosphere) are appropriate. View culture periodically to check the condition. Increase time in culture after recovery.|
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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