Methanococcus maripaludis Jones et al.
BAA-2049 ™
- Product category
-
Archaea
- Product type
-
Extremophile
- Strain designation
- LL [OCM 803]
- Type strain
- No
- Isolation source
- Intertidal sediments
- Product format
- Frozen
- Storage conditions
-
-80°C or colder
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
EXPLOSION HAZARD - We recommend growing cultures in pressure-resistant Balch tubes* to reduce this risk. The cultures should be vented regularly to reduce the gas and prevent overpressure. If it is necessary to grow larger batches of methanol-utilizing methanogens in sealed serum vials, extra caution should be taken. Typically, 3 moles of methane are produced from one mole of methanol.Always wear protective eye wear when working with methanogens growing in tubes or bottles.*Balch tube refers to a special type of test tube that is designed to be pressurized and suited for anaerobic work.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Preceptrol
- No
Characteristics
- Extremophile type
-
Methanogen
- Comments
-
This item is part of The Oregon Collection of Methanogens (OCM) that was kindly donated to the ATCC by Dr. David Boone. This collection is now part of the ATCC general collection and available to the scientific community. OCM items are archived as original material and available on a "made-to-order" basis. ATCC will make a reasonable effort to recover these items from preservation, but recovery and identification is not guaranteed.
Handling information
- Medium
- Temperature
-
37°C
- Atmosphere
-
80% H2, 20% CO2
- Handling procedure
-
1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and then flaming the top.
2. If needed, exchange the gas in the test tube for 80% H2 20% CO2. Do not over pressurize (5 psi until the culture has been inoculated).
3. If the medium is pink (see discussion about resazurin), add 0.2 ml reducing agent (see note Reducing agents below) per 10 ml of medium. Let the medium sit at room temperature for minimum of 30 to 40 minutes (until the resazurin becomes colorless) before inoculating.
4. When the Balch tube is ready to inoculate, thaw the frozen vial at room temperature under a gentle stream of oxygen-free gas.
5. For inoculation, use an anaerobic (see ?c? below) 1.0 ml syringe tipped with 22-gauge needle, withdraw the cell suspension from the vial and transfer it to the broth. Additional tubes of MSH can be inoculated by transferring 0.5 ml of the primary broth using good anaerobic technique. Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37°C. Use 0.1 ml of the inoculated culture to inoculate a nonselective aerobic broth. Incubate the non-selective aerobic broth tubes at 37°C. Increase the head space pressure to 20 psi and incubate the anaerobic tubes at 37°C.
6. Growth should be detected in the MSH broth within 24 to 48 hours. There should be no growth detected on the aerobic plate or in the aerobic broth.
ANAEROBIC CONDITIONS:
a. Balch tube refers to a special type of test tube that is designed to be pressurized and is suited for anaerobic work. The Balch test tubes can be purchased form Bellco glass (www.bellcoglass.com; stock no. 2048-00150).
b. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added. Common reducing agents are sodium sulfide, 3% cysteine, or 1.5% sodium sulfide (stock solutions), Add 0.1 ml of reducing agent for each 10 ml of medium. For BAA-2049?, sodium sulfide worked well as a reducing agent.
c. Syringes can be made anaerobic by one of two methods.
1. Displace the dead space in the syringe with a sterile oxygen-free gas
2. Displace the dead space in the syringe with a reducing agent. - Handling notes
-
Cells are sarcina in shape and occur singly, in pairs, and in clumps; growth settles to the bottom of the tube. No growth should occur on non-selective media either aerobically or anaerobically.
History
- Depositors
- D Boone
- Type of isolate
- Environmental
- Special collection
-
Oregon Collection of Methanogens
- Cross references
-
GenBank CP002913.1 Complete genome sequence
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Boone DR, et al. Diffusion of the interspecies electron carriers H2 and formate in methanogenic ecosystems and its implication in the measurement of Km for H2 or formate uptake. Appl. Environ. Microbiol. 55: 1735-1741, 1989.
Brockman F. Methanosarcina Culture Warning. ASM News 70: 47, 2004.
Sowers KR, Schreier HJ (Editors). Archaea: A laboratory manual, Cold Spring Harbor, NY: CSHL Press, 1995.
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