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Borrelia carolinensis Rudenko et al.

BAA-1773

Borrelia carolinensis strain SCW22 is a bacterial type strain that was isolated in South Carolina from a hard tick.
Product category
Bacteria
Classification
Bacteria, Spirochaetota, Spirochaetia, Spirochaetales, Borreliaceae, Borreliella
Strain designation
SCW22
Type strain
Yes
Isolation source
Hard tick (Ixodes minor); isolated by JH Oliver, Jr
Geographical isolation
United States; South Carolina
Applications
Infectious disease research
Vector-borne disease research
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Handling information

Medium
Temperature
33°C
Atmosphere
95% Air, 5% CO2
Handling procedure
  1. Open thawed vial.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #1914 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #260 plate and/or agar slant.
  4. Or, to obtain a biphasic culture, add several drops of the primary broth tube to an agar slant. Best practice is to incubate these slants at an angle.
  5. Incubate at 33°C in the dark in an atmosphere of 5% CO2 for 7-10 days. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.
Handling notes

Growth usually occurs after 5 days, although some strains may take up to 10 days to grow.  Acid formation during growth will change the medium to a light or yellowish-orange color.  Turbidity is not evident.  Cells can be monitored under phase microscopy as long spiral rods and motility by twitching movement.

Borrelia carolinensis is a fragile, sensitive organism that must have the appropriate medium for growth.  Rabbit serum is essential for the growth of this organism.  Fresh medium enhances growth.  Medium older than one month should not be used.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Depositors
N Rudenko and JH Oliver, Jr
Type of isolate
Arthropod
Year of origin
1994
Cross references
GenBank EU085407 Borrelia carolinensis isolate SCW-22 16S ribosomal RNA (rrs) gene, partial sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Rudenko N, Golovchenko M, Grubhoffer L, Oliver JH Jr. Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi Sensu Lato complex from the southeastern region of the United States. J Clin Microbiol 47(1): 134-141, 2009. PubMed: 19020062

Rudenko N, et al. Borrelia carolinensis sp. nov., a novel species of the Borrelia burgdorferi sensu lato complex isolated from rodents and a tick from the south-eastern USA. Int J Syst Evol Microbiol 61(2): 381-383, 2011. PubMed: 20305062

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