Clostridium acidisoli Kuhner et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of #1053 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053 may also be inoculated with 0.2 ml.
4. Incubate tubes under an anaerobic atmosphere at 30oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system
hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic
gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that
an anaerobic gas headspace is retained.
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Kuhner CH, et al. Clostridium akagii sp. nov. and Clostridium acidisoli sp. nov.: acid-tolerant, N2-fixing clostridia isolated from acidic forest soil and litter. Int. J. Syst. Evol. Microbiol. 50: 873-881, 2000. PubMed: 10758899
acidic peat bog soil, Fichtelgebirge