Clostridium algidixylanolyticum Broda et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant can be inoculated with 0.3 ml. Inoculate several blood agar plates to check for colonial morphology and purity.
4. Incubate tubes and one blood plate under anaerobic conditions at 26oC. Incubate the second plate aerobically as a contamination check.
5. Within 24 to 48 hours, growth is evident by good turbidity and gas in the broth, and gas production is evident on the agar slant. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system
hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw-caps on test tubes in anaerobic chamber,
· Loose screw-caps on test tubes in an activated anaerobic
gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Colonies on #260 medium are b-hemolytic, white, circular, convex, entire, and shiny.
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Broda DM, et al. Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotropic clostridia associated with 'blown pack' spoilage of vacuum-packed meats. J. Appl. Microbiol. 88: 107-116, 2000. PubMed: 10735249
Broda DM, et al. Clostridium algidixylanolyticum sp. nov., a psychrotolerant, xylan-degrading, spore-forming bacterium. Int. J. Syst. Evol. Microbiol. 50: 623-631, 2000. PubMed: 10758868