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In 24-48 hours, growth is evident by turbidity in the broth and growth on the plate incubated aerobically. No growth should be observed on the aerobic plate.
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Warren YA, Tyrrell KL, Citron DM, Goldstein EJ. Clostridium aldenense sp. nov. and Clostridium citroniae sp. nov. isolated from human clinical infections. J Clin Microbiol. 2006 Jul;44(7):2416-22. PMID: 16825358