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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1306 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1306 agar slant and/or plate.
5. Incubate all tubes and plates at 26°C under an atmosphere of 50% Methane and 50% Air. The gas mixture should be exchanged every 48 to 72 hours until turbidity is detected. Turbidity should be detected in 7-10 days.
Cells are Gram-negative non-motile rods found singly or in shapeless aggregates. Colonies on NMS should have a salmon pigmentation.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Wartiainen I, Hestnes AG, McDonald IR, Svenning MM. Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78 degrees N). Int. J. Syst. Evol. Microbiol. 56(Pt 3): 541-547, 2006. PubMed: 16514024