Human rhinovirus 17 (ATCC® VR-1663)

Classification: Picornaviridae, Enterovirus, Rhinovirus B  /  Product Format: frozen

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Classification Picornaviridae, Enterovirus, Rhinovirus B
Agent Human rhinovirus 17
Strain 33342
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Genome Sequenced Strain

Human rhinovirus 17, complete genome

Comments ATCC® VR-1663™ was treated with diethyl ether at ATCC® to remove Mycoplasma. The previous item number was ATCC® VR-1127™ (NIAID Research Reference Reagent V-106-003-021). Strain 33342 is the prototype strain for Human rhinovirus 17.
Effect on Host
CPE, rounding, sloughing
Recommended Host
H1HeLa (ATCC® CRL-1958™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed throughout

Effect on Host
CPE, rounding, sloughing
Name of Depositor NIAID, ATCC
Source Presumed from throat swab from adult with upper respiratory illness, North Carolina, 1959
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054

Cross References

Nucleotide (GenBank) : EF173420.1 Human rhinovirus 17, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation