Phenol Red ATCC ® PCS-999-001™

Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Phenol red is frequently used in cell culture media as a pH indicator.
Product Format	1.0 mL
Storage Conditions	room temperature

Comments	It is provided as an optional media component when preparing complete growth media as part of an ATCC® Primary Cell Solutions™ system. Phenol red is provided as a concentrated stock solution of 33 mM in water. The final concentration in complete growth medium is 33 µM.

Complete Growth Medium	Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of Phenol Red to complete media. Dilute 1:1000 when using to supplement complete media; i.e., 0.5 mL of phenol red per 500 mL of complete cell-specific media. Tightly cap the bottle (or other vessel) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the vessel. Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

Complete Growth Medium

Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of Phenol Red to complete media. Dilute 1:1000 when using to supplement complete media; i.e., 0.5 mL of phenol red per 500 mL of complete cell-specific media.
Tightly cap the bottle (or other vessel) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the vessel.
Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

Volume	1.0 mL
Sterility Tests	Negative for bacteria, fungi and yeast
Functional Tests	Each lot is assessed for acceptable growth of human primary cells compared to control. A Certificate of Analysis (COA) is available upon request for each lot of Phenol Red.
C of A	Certificate of Analysis

Basic Documentation	Product Sheet Certificate of Analysis SDS

Phenol Red (ATCC^® PCS-999-001^™)