Derivation
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EpH4 1424.1 was derived from BALB/c mouse xenografts initiated with EpH4 cells stably transfected with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD). Cells were rederived from a metastatic tumor to the kidney after selection with 1 mg/mL G418 in complete medium.
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Genes Expressed
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Constitutively activated form of MEK1, expressed
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Cellular Products
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stably expresses Glu-Glu tagged MEKDD, which is a constitutively activated form of MEK1 (Asp218/Asp222 MEK1 phosphorylation site mutant)
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Comments
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EpH4 1424.1 was derived from BALB/c mouse xenografts initiated with EpH4 cells stably transfected with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD). Cells were rederived from a metastatic tumor to the kidney after selection with 1 mg/mL G418 in complete medium.
Activation of MEK1 is mediated through phosphorylation of Ser218 and Ser222 by members of the Raf family of kinases.
This cell line stably expresses constitutively activated form of MEK1(MEKDD), and can be used in MEK-MAPK pathway studies.
This cell line was derived from metastatic tumor and can be used to study breast cancer metastasis.
There are four additional related cell lines: B-MEKDD 116 cell line (ATCC CRL-3069), EpH4 1424 cell line (ATCC CRL-3071), EpH4 1424.2 cell line (ATCC CRL-3210) and EpH4-Ev cell line (ATCC CRL-3063).
The EpH4 1424.1 cell line produces the constitutively activated MEK1 mutant MEKDD which has been tagged with Glu-Glu, verified at ATCC.
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