CCD-186Sk (ATCC® CRL-1563)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: cystic fibrosis

Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease cystic fibrosis
Age 16 years
Gender male
Clinical Data
The tissue donor suffered from cystic fibrosis.
Genes Expressed
Homozygous for the Delta 508 mutation in the CFTR gene.
The tissue donor suffered from cystic fibrosis.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.