To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.

X

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >

X

Skip to Content

Search

Login
|
Create a Profile
|
Quick Order
|
(0 items)
|
USA

Select another country:

|
1.800.638.6597

Vero-SF-ACF (ATCC^® CCL-81.5^™)

Organism: Cercopithecus aethiops /

Vero-SF-ACF ATCC^® CCL-81.5^™

frozen

For-Profit: $645.00 Non-Profit: $645.00

Qty:

Recommended for this product

Permits and Restrictions	View Permits
Organism	Cercopithecus aethiops
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	adult
Applications	detection of verotoxin efficacy testing malaria biology media testing mycoplasma testing substrate testing transfection host detection of virus in ground beef
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy.
Images
Derivation	The parental Vero cell line was initiated from the kidney of a normal adult African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan
Comments	Vero-SF-ACF cell line was derived from the parental Vero cell line (ATCC CCL-81) by adaptation to serum-free and animal component-free medium. The parental Vero cell line has been shown to be susceptible to infection by: poliovirus 1, 2, 3; Getah; Ndumu; Pixuna; Ross River; Semliki Forest; Paramaribo; Kokobera; Modoc; Murutucu; Germiston; Guaroa; Pongola; Tacaribe; SV-5; SV40; rubeola; rubellavirus; reovirus 1, 2, 3; and simian adenoviruses. The parental Vero cell line has been show to be resistant to infection by the following viruses: Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa

Complete Growth Medium	These cells may be grown in NutriVero Flex10 (Biological Industries, Cat # 05-068-1A); plus 4mM L-Glutamine (ATCC 30-2214).
Subculturing	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS). Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of 0.25% Soybean Trypsin Inhibitor and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C Note: Cells grow best after the first subculture. For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 6th edition, published by Wiley-Liss, N.Y., 2010. Cell density: The recommended start-up seeding range is 3.0 x 10⁴ to 5.0 x 10⁴ viable cells/cm² and the recommended subculture seeding range is 2.0 x 10⁴ to 5.0 x 10⁴ viable cells/cm². Medium Renewal: One to two times weekly
Cryopreservation	Freeze medium: Stem Cell Freezing Medium (ATCC ACS-3020) Note: Cell counts to determine freeze volume must be taken prior to centrifugation as the freeze medium is not compatible with trypan blue cell counting equipment. Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37.0°C

Name of Depositor	ATCC
Deposited As	Cercopithecus aethiops
Year of Origin	2011
References	Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture - preliminary step for cancer research in vitro. Nihon Rinsho 21: 1201-1215, 1963. Simizu B, et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 119-123, 1967. PubMed: 6027511 Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492 Rhim JS, et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. PubMed: 5689485 Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683 Rhim JS, et al. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. Gesamte Virusforsch. 21: 243-252, 1967. PubMed: 5591575 Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Other Documentation	Cell Micrograph
References	Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture - preliminary step for cancer research in vitro. Nihon Rinsho 21: 1201-1215, 1963. Simizu B, et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 119-123, 1967. PubMed: 6027511 Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492 Rhim JS, et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. PubMed: 5689485 Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683 Rhim JS, et al. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. Gesamte Virusforsch. 21: 243-252, 1967. PubMed: 5591575 Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.