Borrelia coriaceae Johnson et al.

1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions. 
2. Aseptically transfer the entire contents to a 5-6 mL tube of #1914 broth. Additional test tubes can be inoculated by transferring one-tenth of the cell suspension to two or three additional tubes of fresh #1914 broth.
3. Incubate at 30°C under microaerophilic conditions for 7-10 days; 6% O₂ is ideal. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes should be incubated with caps loosened.

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