Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

Permits and Restrictions

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Formulation Formulation
Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications

Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

Comments
NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiency. Additional test results are listed in the Certificate of Analysis.

Test*

Specification

pH

7.0 to 7.4

Osmolality

235 to 309 mOsm/kg

Sterility Testing

Pass

Endotoxin

≤ 0.5 EU/mL

Mycoplasma

Pass

Growth Promotion

Pass

*Please consult the Certificate of Analysis for lot-specific test results.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation