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Nitrobacter winogradskyi Winslow et al.

25391

Nitrobacter winogradskyi strain Nb-255 [1] is a whole-genome sequenced bacterial type strain.
Product category
Bacteria
Product type
Extremophile
Strain designation
Nb-255 [1]
Type strain
Yes
Genome sequenced strain
Yes
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Comments
Genome sequenced strain

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Allow vial to thaw. Transfer the entire contents into 5.0 mL of #480 broth contained in a large test tube (20 X 150 mm).
  2. Incubate tube at 26°C, static, in the dark, and in a slanted position to increase surface area.
  3. Monitor culture growth daily by assaying for the disappearance of NO2 in the culture medium. Withdraw 0.1 mL from the culture and place in a small test tube. Add one drop of NO2 Reagent A followed by one drop of NO2 Reagent B, mixing after each addition. Pink color development occurs within 10 minutes and is directly related to amount of NO2 present. Initial tests will probably be dark magenta reflecting the 3 mM NO2 concentration of medium #480; but as culture grows and NO2 is oxidized, color will become magenta, pink and finally clear. This process can take from 3 to 20 days depending on the viability of the stock.
  4. When all NO2 has been oxidized, inoculate a 250 mL flask containing 100 mL of medium #480 with the 5.0 mL tube culture. Wrap the flask in foil to protect from light and incubate at 26°C with gentle shaking. Monitor NO2 concentration daily.
  5. When NO2 is again depleted in the culture medium, either transfer the culture to fresh media or feed the culture by adding additional NO2. A 10% inoculum is recommended for transfers. To feed cultures, aseptically add sterile NaNO2 solution, returning NO2 concentrations to approximately 10 mM (3.5 mL of 2% NaNO2 per 100 mL culture). Cells should be harvested or sub-cultured to fresh media when cultures can utilize 10 mM NO2 in less than 24 hours and cell numbers are greater than 10 cells per field at 1000X magnification.
  6. Cells can be harvested by centrifugation. For long-term storage, 1.0 mL aliquots of concentrated cells can be frozen with glycerol as the cryoprotectant at a final concentration of 10%. Frozen vial should be stored at -70°C or below for best preservation.
Handling notes

This item does not grow on agar.

When a strain is received as a test tube culture, transfer to fresh media upon arrival. (See step 4.)

NO2 Reagent A:
Sulfanilic acid, 8 g
5N Acetic acid, 1.0 L

NO2 Reagent B:
N,N-Dimethyl-1-naphthylamine, 6.0 ml
5N Acetic acid, 1.0 L

5N acetic acid consists of 1 part glacial acetic acid to 2.5 parts distilled water.
NO2 Reagents A and B may also be purchased from Remel.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Nitrobacter winogradskyi Winslow et al.
Depositors
SW Watson
Chain of custody
ATCC <-- SW Watson <-- A Van Gool <-- Lees 1
Cross references
GenBank CP000115 Nitrobacter winogradskyi Nb-255, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Watson SW. Taxonomic considerations of the family Nitrobacteraceae Buchanan. Requests for opinions. Int. J. Syst. Bacteriol. 21: 254-270, 1971.

Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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