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Nitrosospira multiformis (Watson et al.) Head et al. (ATCC® 25196)

Strain Designations: C 71  /  Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Nitrosolobus multiformis Watson et al.
Strain Designations C 71
Soil, Suriname
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Nitrosospira multiformis ATCC 25196, complete genome.

Type Strain yes
Morphology and DNA content

Genome sequenced strain

When propagating this strain, it is imperative that the medium be prepared according to directions in clean glassware.  Medium may be dispensed into tissue culture flasks rather than glass flasks to avoid trace contaminants sometimes found on glassware.


Medium ATCC® Medium 929: Nitrosolobus medium
Growth Conditions Temperature: 26°C with shaking
Atmosphere: Aerobic 
Name of Depositor SW Watson
Soil, Suriname

Watson SW, et al. A lobular ammonia-oxidizing bacterium Nitrosolobus multiformis nov. sp.. Arch. Mikrobiol. 76: 83-203, 1971. PubMed: 5548293

Watson SW. Taxonomic considerations of the family Nitrobacteraceae Buchanan. Requests for opinions. Int. J. Syst. Bacteriol. 21: 254-270, 1971.

Validation list no. 54. Int. J. Syst. Bacteriol. 45: 619-620, 1995.

Watson SW, Mandel M. Comparison of the morphology and deoxyribonucleic acid composition of 27 strains of nitrifying bacteria. J. Bacteriol. 107: 563-569, 1971. PubMed: 4939767

Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Head IM, et al. The phylogeny of autotrophic ammonia-oxidizing bacteria as determined by analysis of 16S ribosomal RNA gene sequences. J. Gen. Microbiol. 139: 1147-1153, 1993.

Cross References

Nucleotide (GenBank) : L35509 Nitrosolobus multiformis (ATCC 25196) 16S ribosomal RNA (16S rRNA) gene.

Nucleotide (GenBank) : U15733 Nitrosolobus multiformis ammonia monooxygenase operon copy 2: ammonia monooxygenase subunit AmoC2 (amoC2) gene, partial cds, ammonia monooxygenase subunit AmoA2 (amoA2) gene, complete cds and ammonia monooxygenase subunit AmoB2 (amoB2) gene,

Nucleotide (GenBank) : U89833 Nitrosolobus multiformis ammonia monooxygenase operon copy 3: ammonia monooxygenase subunit AmoC3 (amoC3) gene, partial cds, ammonia monooxygenase subunit AmoA3 (amoA3) gene, complete cds and ammonia monooxygenase subunit AmoB3 (amoB3) gene,

Nucleotide (GenBank) : CP000103 Nitrosospira multiformis ATCC 25196, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation