To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at For Technical questions please contact Thank you.

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >

Geobacillus stearothermophilus (Donk) Nazina et al. (ATCC® 12980)

Genome Data Seal Click here to learn about our Enhanced Authentication Initiative

Strain Designations: NCA 26  /  Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Deposited As Bacillus stearothermophilus Donk
Verified By Whole-genome Sequencing
Strain Designations NCA 26
Produces restriction endonuclease BstPI
Steam sterilization control
Sterility assurance
Pharmaceutical and Personal Care
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain yes
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Name of Depositor Nat. Canners Assn.

Can. J. Microbiol. 6: 557, 1960.

J. Gen. Microbiol. 34: 270, 1964.

Pugatsch T, Weber H. A thermostable, sequence-specific restriction endonuclease from Bacillus stearothermophilus: BstPI. Nucleic Acids Res. 7: 1429-1444, 1979. PubMed: 503858

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Nazina TN, et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and .... Int. J. Syst. Evol. Microbiol. 51: 433-446, 2001. PubMed: 11321089

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.

Cross References

Nucleotide (GenBank) : U34874 Bacillus stearothermophilus levansucrase (surB) gene, complete cds.

Nucleotide (GenBank) : X93600 B.stearothermophilus aspC gene.

Nucleotide (GenBank) : M57457 B.stearothermophilus alpha amylase gene, complete cds.

Nucleotide (GenBank) : U34875 Bacillus stearothermophilus levanase (surC) gene, complete cds.

Nucleotide (GenBank) : M63307 Bacillus stearothermophilus histidine utilization operon (hut) gene sequence.

Nucleotide (GenBank) : M26646 B.stearothermophilus manganese superoxide dismutase (MnSOD) gene, complete cds.

Nucleotide (GenBank) : AF055578 Bacillus stearothermophilus surface layer protein precursor (sbsC) gene, complete cds.

Nucleotide (GenBank) : AF139535 Bacillus stearothermophilus trp RNA-binding attenuation protein (mtrB) gene, complete cds.

Nucleotide (GenBank) : AF162268 Bacillus stearothermophilus insertion sequence ISBst12 putative transposase gene, complete cds.

Nucleotide (GenBank) : AF139534 Bacillus stearothermophilus chorismate mutase (aroH), anthranilate synthase subunit (trpE), and anthranilate phophoribosyl transferase (trpD) genes, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019