Neisseria gonorrhoeae (Zopf) Trevisan
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2. Using a single tube of #814 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Incubate all tubes and plate at 37oC with 5% CO2 for 48 hours. Assure that screw caps are loose during incubation period.
Increased CO2 enhances growth.
Brain Heart Infusion (BD 237500) can be used to rehydrate vial contents if #814 broth is not available, but it will not support growth as well as the GC broth.
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