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Clostridioides difficile (Prevot) Lawson et al.

9689-FZ

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Type strain. Produces cytotoxin.
Product category
Bacteria
Product type
Anaerobe
Strain designation
90556-M6S
Applications
Bioinformatics
Enteric disease research
Infectious disease research
Water testing
Product format
Frozen
Storage conditions
-80°C or colder
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Price: $550.00 EA
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces cytotoxin
Enteric Research
Preceptrol
No

Characteristics

Genotype
Toxinotype: 0
Toxigenic
Yes
Toxin genes
cdtB (Binary toxin) negative
tcdA (Toxin A) positive
tcdB (Toxin B) positive
Comments
Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Thaw the frozen vial under anaerobic conditions. 
  2. Under anaerobic conditions, transfer the entire contents of the thawed vial into a single tube (5 - 6 mL) of #2107 broth.  Mix well. 
  3. Additional tubes of broth may be inoculated with 0.5 mL each from the suspension and 0.1 mL may be inoculated onto a slant.  Streak several blood plates for colony morphology and purity.
  4. Incubate the tubes and plate under anaerobic conditions at 37°C for 48 hours. Incubate the purity plate aerobically at 37°C. Turbid growth is evident in the broth at 48 hours.

 

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or
  • Placement of test tubes under a gassing cannula system connected to anaerobic gas.

 

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber,
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes

Colonies on Brucella blood agar are irregular, raised, undulate and gray in color. No growth should occur on the aerobic blood plate.

The presence of tcdA and tcdB genes is confirmed by PCR. The binary toxin gene cdtB is not amplified by PCR.

Purified genomic DNA of this strain is available as ATCC® 9689D-5.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Clostridium difficile (Hall and O'Toole) Prevot
Depositors
FE Clark
Chain of custody
ATCC <-- F E Clark
Special collection
NSF - Bacteriology
Cross references
GenBank X94328 C.difficile spo0A gene.
GenBank AX110003 Sequence 736 from Patent WO0123604.
GenBank AX110007 Sequence 740 from Patent WO0123604.
GenBank AX110095 Sequence 828 from Patent WO0123604.
GenBank AX111484 Sequence 2217 from Patent WO0123604.
GenBank AX111505 Sequence 2238 from Patent WO0123604.
GenBank AX111525 Sequence 2258 from Patent WO0123604.
GenBank U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds.
GenBank S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt]
GenBank U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634

Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

View All Curated Citations for this Product

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