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Clostridium acetobutylicum McCoy et al. emend. Keis et al.


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Clostridium acetobutylicum is a whole-genome sequenced type strain. This bacterial culture degrades xylan and produces acetone, acidolysin, butyl alcohol [butanol], autobacteriocin, and restriction endonuclease Cac8241.
Product category
Product type
Strain designation
[CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787]
Type strain
Genome sequenced strain
Isolation source
Plant-derived foodstuff - cornmeal
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of 4-aminobenzoic acid p-aminobenzoic acid
Degrades xylan
Produces acetone
Produces acidolysin
Produces autobacteriocin
Produces butyl alcohol butanol
Produces ethyl alcohol ethanol
Produces restriction endonuclease Cac824I
Transformation host


Genome sequenced strain
Verification of identity

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
  3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
  4. Incubate in an anaerobic atmosphere at 37°C for 24-48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

  • Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
  • Loose screw caps on test tubes in an anaerobic chamber
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
Purified genomic DNA of this strain is available as ATCC® 824D-5™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Granulobacter pectinovorum (Stormer) Beijerinck
ER Weyer
Chain of custody
ATCC <-- ER Weyer
Type of isolate
Food & Beverage
Cross references
GenBank X78070 C. acetobutylicum (ATCC 824, T) 16S rRNA
GenBank X70334 C. acetobutylicum gene for starch degrading enzyme
GenBank M68865 Clostridium acetobutylicum autolytic lysozyme (lyc) gene, complete cds
GenBank AF205034 Clostridium acetobutylicum sucrose utilization operon, complete sequence.
GenBank M96945 Clostridium acetobutylicum butanol dehydrogenase isozyme A gene, complete cds
GenBank M96946 Clostridium acetobutylicum butanol dehydrogenase isozyme B gene, complete cds
GenBank AE001438 Clostridium acetobutylicum ATCC 824 megaplasmid pSOL1, complete sequence
GenBank S77333 thl=thiolase {5' region} [Clostridium acetobutylicum, ATCC 824, Genomic, 81 nt]
GenBank U16166 Clostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequence
GenBank AF047822 Clostridium acetobutylicum catabolic acetolactate synthase (alsS) gene, complete cds
GenBank U38234 Clostridium acetobutylicum phosphotransacetylase (pta) and acetate kinase (ack) genes, complete cds
GenBank AF321779 Clostridium acetobutylicum plasmid pSOL1 aldehyde/alcohol dehydrogenase (adhE2) gene, complete cds
GenBank L14744 Clostridium acetobutylicum phosphotransbutyrylase, complete cds; butyrate kinase, complete cds and ORF1
GenBank L14817 Clostridium acetobutylicum transcriptional repressor (solR) and aldehyde-alcohol dehydrogenase (aad) genes, complete cds
GenBank U52366 Clostridium acetobutylicum phosphofructokinase (pfk) gene, complete cds, and pyruvate kinase homolog (pyk) gene, partial cds
GenBank M93363 Clostridium acetobutylicum acetoacetate decarboxylase, acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase subunit, complete cds
GenBank U08465 Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase gene, complete cds, and homolog of B. subtilis orfX gene, partial cds
GenBank U15277 Clostridium acetobutylicum putative regulatory protein and putative ortho phosphoribosyl transferase (pyrE) genes, partial cds and hydrogenase I (hydA) gene, complete cds
GenBank U52368 Clostridium acetobutylicum rubredoxin oxidoreductase homolog and deformylase (def) genes, complete cds, and methionyl-tRNA formyltransferase homolog (fmt) gene, partial cds
GenBank U07420 Clostridium acetobutylicum ATCC 824 putative sigma factor E (sigE) gene, putative sigma factor E processing enzyme (ORFA), gene and putative sigma factor G (sigG) gene, complete cds
GenBank U52367 Clostridium acetobutylicum ATCC 824 F-type ATP synthase subunit a (atpA) gene, F-type ATP synthase subunit c (atpC) gene, and F-type ATP synthase subunit b (atpB) gene, complete cds
GenBank U17110 Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA dehydrogenase
GenBank AE001437 Clostridium acetobutylicum ATCC 824, complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Lee SF, et al. Xylanolytic activity of Clostridium acetobutylicum. Appl. Environ. Microbiol. 50: 1068-1076, 1985.

Soucaille P, Goma G. Acetonobutylic fermentation by Clostridium acetobutylicum ATCC 824: Autobacteriocin production, properties, and effects. Curr. Microbiol. 13: 163-169, 1986.

Croux C, et al. Purification and characterization of acidolysin, an acidic metalloprotease produced by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 56: 3634-3642, 1990. PubMed: 2082818

Validation list no. 51. Int. J. Syst. Bacteriol. 44: 852, 1994.

J. Infect. Dis. 56: 333-346, 1935.

View All Curated Citations for this Product

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