Thiobacillus thioparus Beijerinck
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2. Withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional broth and slant tubes may be inoculated with 0.3 ml each from the initial broth tube. Agar plates may be inoculated using 0.1 ml of the initial broth per plate.
4. Incubate tubes and plates at 26oC.
5. After approximately 7 days of incubation, growth should be evident by turbidity in the broth and by colonies on the agar surfaces.
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Adachi K, Suzuki I. Purification and properties of glutamate synthase from Thiobacillus thioparus. J. Bacteriol. 129: 1173-1182, 1977. PubMed: 14919
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.