Helicobacter canadensis Fox et al.


Type strain. Resistant to cephalothin and nalidixic acid. This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.
Product category
Product type
Drug-resistant bacterium
Strain designation
NLEP 16143 [98-5491, MIT98-5491]
Type strain
Genome sequenced strain
Antimicrobial resistance research
Drug development
Product format
Storage conditions
2°C to 8°C
Mission Collection Item
This is a Mission Collection Item


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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




Susceptibility profile
Nalidixic Acid
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.

Handling information

Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
  1. Open vial according to enclosed instructions. 
  2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
  3. To obtain a biphasic culture, add 0.4 mL of the suspension to a #260 slant.  Add remaining 0.1 mL of the suspension to a #260 plate and streak for isolation.
  4. Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.  Incubate slant with cap loose for three days.
  5. Within three days of incubation, good growth should be obtained in the broth pool at the bottom of the slant. Additional incubation may be required for colonies to appear on the plate.  Further subcultures can be made using broth pool as the inoculum source.
Handling notes
This is a slow growing organism that requires moist conditions for best growth.  Growth at the broth/agar interface of the biphasic slant should occur within three days, but little turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy.  The organism is a medium size, regular to slightly curved, motile bacillus. Motility is usually observed only in young cultures. The presence of spheroid cells indicates that viability is being lost either due to age or too much exposure to oxygen.

Growth on agar takes longer than with the biphasic culture.  Colonies are small, circular, entire, and gray.  Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.  Viability also decreases with repeated subculturing. The cells do not Gram stain well using traditional procedures. To obtain the best results, use a basic fuchsin counterstain in place of the safranin.

Once good growth is obtained, transfer or freeze the culture.  Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or "ultra-low temperature" freezer is recommended.

Additional information on this culture is available on the ATCC® web site at


Deposited as
Helicobacter canadensis Fox et al.
JG Fox
Chain of custody
ATCC <-- JG Fox <-- P. Melito, D. Woodward, F. Rogers
Special collection
NCRR Contract
Human Microbiome Project (HMP)
Cross references
GenBank AF262037 Helicobacter canadensis 16S ribosomal RNA gene, partial sequence.
GenBank ABQS01000000 Helicobacter canadensis MIT 98-5491, whole genome shotgun sequencing project

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Fox JG, et al. Helicobacter canadensis sp. nov. isolated from humans with diarrhea as an example of an emerging pathogen. J. Clin. Microbiol. 38: 2546-2549, 2000. PubMed: 10878041

Validation list no. 84. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 52: 3-4, 2002. PubMed: 11837316

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