Glaciecola pallidula Bowman et al.
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2. From a tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 with a sterile pipette; add this medium to the vial to rehydrate the pellet.
3. Aseptically transfer the contents of the vial into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate all tubes and plate at 10°C for 4-6 days.
Colonies are circular, convex, entire, and yellow.
In broth, growth was observed from 4-26oC. On solid media, growth takes 10 days.
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Bowman JP, et al. Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int. J. Syst. Bacteriol. 48: 1213-1222, 1998.
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