Methylopila capsulata Doronina et al.
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2. Using a single tube of broth, aseptically transfer 0.5 ml to the vial and rehydrate the pellet.
3. Aseptically transfer this aliquot back to the broth tube and mix well. Transfer 0.1 ml to slants. Plate the rehydrated culture (0.1 ml ) onto non selective medium.
4. Incubate the culture at 30oC for three to five days.
Under microscopic examination, the cells are motile rods, in singles and pairs. They are Gram negative, non-pigmented cells, and endospores are absent.
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Doronina NV, et al. Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacterium. Int. J. Syst. Bacteriol. 48: 1313-1321, 1998. PubMed: 9828432