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Bartonella koehlerae Droz et al.


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Product category
Strain designation
Type strain
Genome sequenced strain
Isolation source
Geographical isolation
United States; California
Infectious disease research
Vector-borne disease research
Product format
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




Cross-referenced with BAA-2249

Handling information

Handling procedure


1.    Thaw frozen vial of culture.

2.    Transfer 0.l ml of culture to a fresh #2119 plate aseptically.  Streak for isolation.

3.    The remaining content in the vial can be used to inoculate additional #2119 plates or slants.

4.    Incubate plates under microaerophilic condition, at 35-37oC.

5.    Growth should occur after 7 to 10 days.

Handling notes
This organism is very difficult to cultivate.  The medium has to be fresh, less than 7 days old.  It is crucial that ingredients from Acumedia are used, and the plates are poured with 28 ml of medium/plate.  This organism will grow on Chocolate agar (#814) with 28 ml of medium/plate, but poor growth is observed.  The organism dies rapidly at refrigeration temperature; do not store it at 4oC.  The best way of long-term preservation is under liquid nitrogen atmosphere.  Use the following freezing medium for preservation:

M199 liquid (Gibco, Cat. No. 11150), 500.0 ml

Sodium pyruvate (11g/L), 5.0 ml

Glutamine (200 mM), 5.0 ml

Fetal bovine serum, 100.0 ml

DMSO, 6.1 ml

Mix and filter sterilize using 0.2 um filter.  Mix freshlygrown culture in the freezing medium. 

 Store at -80oC or in liquid nitrogen.

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Bartonella koehlerae Droz et al.
JE Koehler
Type of isolate
Cross references
GenBank AF076237 16S rDNA sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Droz S, et al. Bartonella koehlerae sp. nov., isolated from cats. J. Clin. Microbiol. 37: 1117-1122, 1999. PubMed: 10074535

Validation list No. 73. Int. J. Syst. Evol. Microbiol. 50: 423-424, 2000.

type strain

blood of a healthly 6-month-old stray farm kitten, Greater San Francisco Bay area

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