Ammoniphilus oxalivorans Zaitsev et al.
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2. Using a single tube of #2103 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Incubate tube at 30°C for 72 to 96 hours.
5. Transfers may now be made to a second tube of broth, a slant, and/or plate.
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Zaitsev GM, et al. New aerobic ammonium-dependent obligately oxalotrophic bacteria: description of Ammoniphilus oxalaticus gen. nov., sp. nov. and Ammoniphilus oxalivorans gen. nov., sp. nov.. Int. J. Syst. Bacteriol. 48: 151-163, 1998. PubMed: 9542085