Alkalibacillus haloalkaliphilus (Fritze) Jeon et al.
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2. Using a single tube of broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Incubate tube at 37°C for 24 to 48 hours with shaking.
5. Transfers may now be made to a second tube of broth, a slant, and/or plate.
Colonies are circular, flat, entire, cream, with no pigments present.
This organism is an obligate alkaliphile and can tolerate high concentrations of salt.
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Fritze D. Bacillus haloalkaliphilus sp. nov.. Int. J. Syst. Bacteriol. 46: 98-101, 1996.