Marichromatium purpuratum (Imhoff and Truper) Imhoff et al.
700430 ™
- Product category
-
Bacteria
- Product type
-
Extremophile
- Strain designation
- 95CAR100a
- Type strain
- No
- Isolation source
- Caribbean Sea copepod enrichments, 100 m
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Preceptrol
- No
Characteristics
- Extremophile type
-
Phototroph
Handling information
- Medium
- Temperature
-
28°C
- Atmosphere
-
Anaerobic
- Incubation
- Under light
- Handling procedure
-
1. Put 6 to 8 ml of Medium #2112 into a 13 x 100 mm screw cap test tube (small). Add 3.0 % cysteine (stock concentration, 2 ml/100 ml medium) and then fill the test tube to capacity with Medium #2112. Seal the test tube with a screw cap.
2. Let the tube sit at room temperature for 30 minutes before inoculating. Five minutes prior to inoculating the tube of pre-reduced medium, let the liquid nitrogen vial thaw by placing it at room temperature.
3. Aseptically transfer 1.0 ml of the pre-reduced medium to a sterile test tube. Take the thawed liquid nitrogen vial and aseptically transfer the cells to the tube of pre-reduced medium, fill the tube to capacity with the 1.0 ml of medium that was previously transferred to the sterile test tube. Seal the tube and incubate at room temperature under a tungsten lamp.
4. Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (1020%) the medium does not need to be pre-reduced.
5. When examined microscopically, the cells are fat some times long rods that occur singly and in pairs. The cells are motile.
- Handling notes
-
This culture is tolerant to oxygen therefore strictly anoxic conditions are not required when thawing the liquid nitrogen vial or when transferring the organism.
When grown anaerobically in broth the culture is purple in color.
This culture is able to grow aerobically on agar (Nutrient) in the dark. Colonies are rounded, moist, entire with clear edges and dark red centers.
History
- Deposited as
- Chromatium purpuratum Imhoff and Truper
- Depositors
- LM Proctor
- Type of isolate
- Environmental
- Year of origin
- 1995
- Cross references
-
GenBank AF001581 16S rRNA sequence
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Imhoff JF, et al. Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int. J. Syst. Bacteriol. 48: 1129-1143, 1998. PubMed: 9828415
Lita M Proctor, personal communication
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