Bacillus vedderi Agnew et al.
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2. Using a single tube of #2058 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30°C for 24-48 hours.
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Validation list no. 56. Int. J. Syst. Bacteriol. 46: 362-363, 1996.
Agnew MD, et al. Isolation and characterization of novel alkaliphile from bauxite-processing waste and description of Bacillus vedderi sp. nov., a new obligate alkaliphile. Syst. Appl. Microbiol. 18: 221-230, 1995.