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Staphylococcus aureus subsp. aureus Rosenbach

6538P

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Used in media and sterility testing. Used in the assay of neomycin, cefactor, amikacin, kanamycin, cefoxitin, cefazolin, nafcillin, oxacillin, vancomycin, lincomycin, penicillin, framycetin, tobramycin, spiramycin, gramicidin, cefadroxil, cefotaxime, cephalexin, cephapirin, cephradine, plicamycin [mithramycin], gentamicins [gentamicin], doxycycline [hydramycin], methicillin, cefamandole, cephalothin, cloxacillin, cycloserine [D-4-amino-3-isoxazolidone], erythromycin, tetracycline, meclocycline, methacycline, cephaloglycin, cephaloridine [cephalomycin], dicloxacillin, demeclocycline [demethylchlortetracycline], oxytetracycline [terramycin], rolitetracycline, and chlortetracycline [aureomycin]. Used in susceptibility disc testing of kanamycin, clindamycin, streptomycin, and tetracycline.
Product category
Bacteria
Strain designation
FDA 209P
Type strain
No
Applications
Enteric disease research
Infectious disease research
Media testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Assay of amikacin
Assay of cefactor
Assay of cefadroxil
Assay of cefamandole
Assay of cefazolin
Assay of cefotaxime
Assay of cefoxitin
Assay of cephalexin
Assay of cephaloglycin
Assay of cephaloridine cephalomycin
Enteric Research
Assay of cephalothin
Assay of cephapirin
Assay of cephradine
Assay of chlortetracycline aureomycin
Assay of cloxacillin
Assay of cycloserine D-4-amino-3-isoxazolidone
Assay of demeclocycline demethylchlortetracycline
Assay of dicloxacillin
Assay of doxycycline hydramycin
Assay of erythromycin
Assay of framycetin
Assay of gentamicins gentamicin
Assay of gramicidin
Assay of kanamycin
Assay of lincomycin
Assay of meclocycline
Assay of methacycline
Assay of methicillin
Assay of nafcillin
Assay of neomycin
Assay of oxacillin
Assay of oxytetracycline terramycin
Assay of penicillin
Assay of plicamycin mithramycin
Assay of rolitetracycline
Assay of spiramycin
Assay of tetracycline
Assay of tobramycin
Assay of vancomycin
Control strain
Media testing
Sterility testing
Susceptibility disc testing clindamycin
Susceptibility disc testing kanamycin
Susceptibility disc testing streptomycin
Susceptibility disc testing tetracycline
Susceptibility testing
Testing
Preceptrol
No

Characteristics

Comments
ATCC 6538P was found moderately resistant to penicillin in 1944 using a Penicillin P2 disk. This result was confirmed in initial characterization at ATCC, but subsequent investigation using a Penicillin P10 disk did not show resistance.  Resistance has not been confirmed using modern methods in accordance with the Clinical & Laboratory Standards Institute guidelines.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #117 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #117 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #117 plate and/or #117 agar slant.
  4. Incubate at 37°C for 18 to 24 hours.
Handling notes

ATCC 6538P was found moderately resistant to penicillin in 1944 using a Penicillin P2 disk. This result was confirmed in initial characterization at ATCC, but subsequent investigation using a Penicillin P10 disk did not show resistance.  Resistance has not been confirmed using modern methods in accordance with the Clinical & Laboratory Standards Institute guidelines.

For best results, propagate on ATCC Medium #117. Growth on alternate media may result in the loss of traits such as antibiotic resistance. According to the depositor, this strain has demonstrated resistance to Penicillin using the P2 antibiotic disk.

Strain is coagulase positive and β-lactamase negative.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Staphylococcus aureus subsp. aureus Rosenbach
Depositors
FDA

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

European Pharmacopoeia Commission Sterility. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.1, 1997

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (total viable aerobic count). Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.12, 1997

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

View All Curated Citations for this Product

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