Listeria monocytogenes (Murray et al.) Pirie
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
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Gilot P, Andre P. Characterization of five esterases from Listeria monocytogenes and use of their electrophoretic polymorphism for strain typing. Appl Environ Microbiol 61: 1661-1665, 1995. PubMed: 7747982