Acidithiobacillus caldus (Hallberg and Lindstrom) Kelly and Wood
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2. Using a single tube of #1995 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a non selective agar to check for contamination.
5. Incubate the tubes and plate at 45°C for 48 to 72 hours.
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Hallberg KB, Lindstrom EB. Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile. Microbiology 140: 3451-3456, 1994. PubMed: 7533596